RIEGL USA will be exhibiting at the 2014 AGU Fall Meeting! The AGU 2014 Fall Meeting will be held at the Moscone Convention Center in San Francisco, California from December 15 through December 19, 2014. RIEGL USA will be exhibiting at Booth 2338 during the conference.

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Please be sure to come by our booth to talk to our staff about any questions that you may have about our hardware and software solutions! Be sure to stop by the Moscone West Poster Hall to see the 'Snow Depth from LiDAR: Challenges and New Technology for Measurements in Extreme Terrain' poster containing data from the RIEGL LMS-Q1560 long-range airborne laser scanner! RIEGL USA is looking forward to a successful conference and to seeing you at the 2014 AGU Fall Meeting! The new video of the brand new Riegl RiCOPTER is now on YouTube! RiCOPTER, Riegl's ready to fly remotely piloted airborne laser scanning system (equipped with the survey grade VUX-1 LiDAR sensor), was unveiled at Intergeo in Berlin last week with great excitement! RiCOPTER is a high-performance UAV which can be equipped with the Riegl VUX-1 survey-grade LiDAR sensor to offer a fully integrated turnkey solution and marks the first complete UAS LiDAR solution from one manufacturer.

The robust and reliable RiCOPTER platform design includes a fully integrated Riegl VUX LiDAR sensor, IMU/ GNSS unit with antenna, control unit, and up to 4 optional cameras providing remarkable measuring characteristics: 330deg Field of View, 500,000 measurements/sec., and 10mm accuracy. The Class 1 unmanned aircraft system can be flown at a max. Operating altitude of 550meters with a maximum take-off mass of up to 25kg, a max payload of 16kg, providing a long flight endurance of 30 minutes. RiCOPTER flight characteristics are smooth and stable in hovering positions, as well as on demanding flight maneuvers under challenging conditions.

The only complete survey grade unmanned scanning platform for a variety of demanding applications, like corridor, power line, or railway mapping on the market is now available! Watch the RiCOPTER flight demonstration video on! Riegl >USA will be exhibiting at GSA 2014! GSA 2014 will be held at the Vancouver Convention Center in Vancouver, British Columbia, Canada from October 19 through October 22, 2014. RIEGL USA will be exhibiting at Booth 1013 during the conference.

Please be sure to come by our booth to talk to our staff about any questions that you may have about our hardware and software solutions! Stop by Booth 1013 to schedule a demonstration of our Riegl VMZ, our hybrid mobile laser mapping system, which will be integrated with our Riegl VZ-1000 terrestrial laser scanner! Riegl USA be attending the 5th International Conference on Remote Sensing in Archaeology! The 5th International Conference on Remote Sensing in Archaeology will be held at Duke University in Durham, North Carolina from October 13 through October 15, 2014. The 5th International Conference on Remote Sensing in Archaeology seeks to explore the age of sensing. RIEGL USA is looking forward to a successful conference and to seeing you at the 5th International Conference on Remote Sensing in Archaeology! Riegl will be attending and exhibiting at the CyArk 500 Annual Summit!

The CyArk 500 Annual Summit will be held at the National Archives Building in Washington, D.C. On October 7 and 8, 2014. The CyArk 500 Annual Summit is the second annual conference with a theme of 'Democratizing cultural heritage: Enabling access to information, technology, and support.' Riegl will have the VZ-400 terrestrial laser scanner on display at the CyArk table. Stop by the CyArk table to see the VZ-400 for yourself and then come by the Riegl table to find out more information on the VZ-400 and to learn more about Riegl's cultural heritage applications. ' Riegl has demonstrated a long-standing commitment to the preservation of cultural heritage sites. Its support of digital preservation of sites such as the Pyramids in Giza, the Sphinx, the Domitilla Catacombs in Rome, Frauenkirche in Dresden, St.

Stephen's Cathedral in Vienna and numerous other significant monuments provides future generations the ability to visit such sites in digital form.' , said Johannes Riegl, Jr., chief marketing officer of Riegl. Riegl is looking forward to a successful partnership with CyArk and to seeing you at the CyArk 500 Annual Summit!

RIEGL USA and technet-rail will be exhibiting at the American Railway Engineering and Maintenance-of-Way Association (AREMA) 2014 Annual Conference and Exposition! AREMA 2014 will be held at the Hilton Chicago in Chicago, Illinois from September 28 through October 1, 2014. RIEGL USA and technet-rail will be exhibiting at Booth 703 during the conference. Please be sure to come by our booth to talk to our experts!

Learn about Riegl's solution for railway mapping and monitoring, the Riegl VMX-450-RAIL: A proven fully integrated, high-speed mobile laser scanning system. It includes special hardware for quick and flexible mounting on railcars and new interfaces to technet-rail's SiRailScan 3D data processing software solution for fast, rail-specific data processing. SiRailScan uses automatic extraction of the as-built track geometry to enable features such as modeling of railway infrastructure and clearance gauge analysis and oversize transport simulation through the utilization of complex train profiles with as build axis-based measurements. Automatic monitoring of contact lines / catenary wire geometry inspection is also part of the portfolio. RIEGL USA and technet-rail are looking forward to a successful conference and to seeing you at AREMA 2014! RIEGL USA will be exhibiting and presenting at the Latin America Geospatial Forum!

The Latin America Geospatial Forum will be held at the Hilton Hotel, Mexico Reforma in Mexico City, Mexico from September 22 through September 25, 2014. RIEGL USA will be exhibiting at Booth 19 during the conference. Please be sure to come by our booth to talk to our staff about any questions that you may have about our hardware and software solutions!

Stop by Booth 19 to see demonstrations of our RIEGL VZ-400 3D terrestrial laser scanner! RIEGL USA will be exhibiting at the International Mining Conference and Expo (IMEX) 2014! IMEX 2014 will be held at the Las Vegas Convention Center in Las Vegas, Nevada from September 23 through September 25, 2014. RIEGL USA will be exhibiting at Booth 635 during the conference. Please be sure to come by our booth to talk to our staff about any questions that you may have about our hardware and software solutions! Stop by Booth 635 to see demonstrations of our Riegl VZ-4000 very long range terrestrial laser scanner!

RIEGL USA will be exhibiting at the Florida Surveying and Mapping Society's (FSMS) 59th Annual Conference! The FSMS 59th Annual Conference will be held at the Sawgrass Marriott Resort and Spa in Ponte Vedra Beach, Florida from August 13 through August 17, 2014. RIEGL USA will be exhibiting at Booth 15 during the conference. Please be sure to come by our booth to talk to our staff about any questions that you may have about our hardware and software solutions!

Stop by our booth to ask about our new RIEGL VMZ, a terrestrial to mobile laser scanning solution! RIEGL USA is looking forward to a successful conference and to seeing you at the FSMS 59th Annual Conference!

RIEGL USA will be exhibiting at IAI's 99th International Education Conference! IAI's 99 th International Educational Conference will be held at the Minneapolis Convention Center in Minneapolis, Minnesota from August 10 through August 16, 2014. RIEGL USA will be exhibiting at Booth 609 during the conference. Please be sure to come by our booth to talk to our staff about any questions that you may have about our hardware and software solutions! RIEGL USA is looking forward to a successful conference and to seeing you at IAI's 99th International Education Conference!

RIEGL was honored to accept the 2014 MundoGEO#Connect Award for Most Memorable Brand of Terrestrial Laser Scanner at the MundoGEO#Connect Latin America 2014 Conference and Trade Fair of Geomatics and Geospatial Solutions, which took place at the Frei Caneca Convention Center in Sao Paulo, Brazil, on May 8th, 2014. The prize was received by Mr. Roger Neves, CEO of RIEGL‘s partner in Brazil, CPE Equipamentos Topograficos Ltda. (www.cpeltda.com.br)'CPE is proud to be the RIEGL dealer in Brazil and is very pleased to see RIEGL getting the deserved market recognition!' Said Roger Neves. 'We are very pleased to receive the MundoGeo award for the most memorable brand, which is an acknowledgment of the outstanding qualities of our VZ-Line terrestrial laser scanners. Congratulations to our partners for building up the RIEGL brand throughout South America!'

Commented Johannes Riegl Jr., RIEGL Chief Marketing Officer. RIEGL will be exhibiting and presenting at the 2014 Esri User Conference! The 2014 Esri User Conference will be held at the San Diego Convention Center in San Diego, California from July 14 through July 17, 2014.

Christian Sevcik, Manager of Strategic Software Alliances from Riegl, will be presenting 'Waveform LiDAR: Advantages, Benefits, and How to Read It in ArcGIS' during the 2014 Esri User Conference. He will be presenting twice during the conference at the Demo Theater at the Imagery Island in Exhibit Hall C. The first presentation will take place on Tuesday, July 15 from 11:30 AM-12:00 PM and the second presentation will take place on Tuesday, July 15 from 3:30 PM-4:00 PM. Riegl will also be exhibiting at Booth 1513 in the Imagery Island during the conference. Please be sure to come by our booth to talk to our staff about any questions that you may have about our hardware and software solutions! The new Riegl VUX-1, the world's first survey-grade LiDAR for UAS and the first instrument of its kind, will also be on display during 2014 Esri User Conference. Riegl is looking forward to a successful conference and to seeing you at the 2014 Esri User Conference!

Riegl distribution network. 'To meet the growing demand of our products in North Africa and the Middle East, we've decided to partner with local experts. We are now very proud to have two recognized experts with their companies representing RIEGL in this area.

Khaled El Nabbout and his company, Mena 3D, as well as Dr. Amer Alroichdi with Mapping Solutions' said Johannes Riegl Jr., Riegl Chief Marketing Officer, after the signing of the distribution contracts in Vienna earlier this month. Operating out of their main offices in Dubai, the new distribution partners will both be in charge of the GCC countries of Saudi Arabia, Oman, the United Arab Emirates, Qatar, Kuwait, and Bahrain. Mapping Solutions (www.mapping-solutions.co.uk) additionally will be responsible for the markets in Egypt, Yemen, Iraq, Jordan, and Syria. Mena 3D (www.mena3d.com) will additionally represent Riegl in Lebanon, Libya, Morocco, and Tunisia. With strong support from Riegl LMS, the two new partners will promote the innovative hard-and software solutions that Riegl is providing for terrestrial, airborne, mobile, and UAS based laser scanning. Riegl is very excited about the partnership with Mena 3D and Mapping Solutions and is looking forward to the expanded presence in the Middle East and North Africa!

RIEGL will be exhibiting and presenting at the 2014 Esri 3D Mapping Forum! The 2014 Esri 3D Mapping Forum will be held at the Omni San Diego Hotel in San Diego, California from July 12 through July 13, 2014. Christian Sevcik, Manager of Strategic Software Alliances from Riegl, will be presenting 'The Nuts and Bolts of LiDAR' on Saturday, July 12, 2014 from 10:00 AM to 11:30 AM. The session will discuss the entire Riegl portfolio and break it down into its nuts and bolts.Topics covered will include: Laser Scanning Technology, Terrestrial and Industrial LiDAR, Mobile LiDAR, Airborne and Bathymetric LiDAR, and the latest Riegl developments, including the UAV markets and VUX-1.

See how Riegl can take you from acquisition to final deliverable in simple steps! Riegl will also be exhibiting at Table M109 during the conference. Please be sure to come by our table to talk to our staff about any questions that you may have about our hardware and software solutions! Riegl is looking forward to a successful conference and to seeing you at the 2014 Esri 3D Mapping Forum >.

RIEGL USA will be exhibiting at the 2014 Joint Navigation Conference! The 2014 Joint Navigation Conference, hosted by the ION Military Division, will be held at the Renaissance Orlando at SeaWorld in Orlando, Florida from June 16 through May 19, 2014. RIEGL USA will be exhibiting at Booth 29 during the conference. Please be sure to come by our booth to talk to our staff about any questions that you may have about our hardware and software solutions! RIEGL USA looks forward to a successful conference and to seeing you at the 2014 Joint Navigation Conference! The RIEGL LMS-Q780 airborne laser scanner was awarded with the Geospatial World Award in the Geospatial Technology Innovation LiDAR category at the Geospatial World Forum Conference in Switzerland. 'We are proud to receive this prestigious award for the LMS-Q780.

We are especially happy to receive our second Geospatial Innovation Award in a row for our airborne products after winning the award for our topo-bathymetric laser scanner, the VQ-820-G, last year' stated Peter Rieger, Riegl's Airborne Laser Scanning Division Manager, who was presented the award during the Award Night and Gala Dinner of the Geospatial World Forum 2014 in Geneva, Switzerland. The RIEGL LMS-Q780 high-performance airborne laser scanner is a versatile laser scanning engine for a variety of applications: from corridor to wide-area and large-scale national mapping. With an operating flight altitude of up to 15,500 feet above ground level, the capability of handling more than 10 pulses in the air simultaneously, and a wide field of view of 60 degrees, the LMS-Q780 accounts for unrivaled, industry leading efficiency in data acquisition. RIEGL USA will be exhibiting and presenting at AUVSI's Unmanned Systems 2014! AUVSI's Unmanned Systems 2014 will be held at the Orange County Convention Center in Orlando, Florida from May 13 through May 15, 2014. RIEGL USA will be exhibiting at Booth 827 during the conference. Please be sure to come by our booth to talk to our staff about any questions that you may have about our hardware and software solutions!

Joshua France, Mobile Systems Segment Manager for RIEGL USA, will be presenting 'Introducing a New Way to Survey: Unmanned Aerial Systems (UAS)' on Tuesday, May 13 from 1:30 PM-2:00 PM in the Exhibit Hall. RIEGL USA looks forward to a successful conference and to seeing you at AUVSI's Unmanned Systems 2014! RIEGL USA will be exhibiting and presenting at the Small Unmanned Systems Business Exposition (sUSB-Expo). SUSB-Expo will be held at The Observation Post at the Presidio in San Francisco, California on May 8 and May 9, 2014. RIEGL USA will be exhibiting during the conference. Please be sure to stop by our table to talk to our staff about any questions that you may have about our hardware and software solutions! Joshua France, Mobile Systems Segment Manager for RIEGL USA, will be presenting 'Introducing a New Way to Survey: Unmanned Aerial Systems (UAS)' on Friday, May 9 from 11:20 AM-11:40 AM.

RIEGL USA looks forward to a successful conference and to seeing you at the sUSB-Expo! RIEGL USA will be exhibiting and presenting at SPIE Defense, Security, and Sensing (DSS) 2014. SPIE DSS 2014 will be held at the Baltimore Convention Center in Baltimore, Maryland from May 5 through May 9, 2014. RIEGL USA will be exhibiting at Booth 636 during the conference. Please be sure to come by the booth to talk to our staff about any questions that you may have about our hardware and software solutions! Martin Pfennigbauer, Director of Research & Intellectual Property for Riegl, will be presenting 'Online waveform processing versus full waveform analysis for demanding target situations' in Session 3: Signal Processing at SPIE DSS 2014.

Pfennigbauer's presentation will be on Wednesday, May 7 from 8:00 AM to 9:40 AM in Convention Center Room 331. After the exceptional success of Riegl L Riegl announces its next International User Conference, Riegl LiDAR 2015, to be held in Hong Kong and Guangzhou, China. 'To meet the growing demand of our products in China, we've opened a new Riegl CHINA office just recently. To recognize this development, we will hold our next user conference in Asia. We look forward to welcoming our international and Chinese community to the spectacular cities of Hong Kong and Guangzhou!'

Said Johannes Riegl Jr., Riegl Chief Marketing Officer. The international conference in Hong Kong, with English as the primary language, will be held Tuesday, May 5 through Thursday, May 7, 2015. The conference in Guangzhou, with Mandarin and English as the main languages, will be held Thursday, May 7 and Friday, May 8, 2015. For every visitor, there will be the option to either participate in the Hong Kong or the Guangzhou part of the conference (or of course in both!), depending on convenience and preference. Riegl USA is exhibiting this week at SPAR International 2014. Find us at Booth 430 and will have presentations throughout the conference. Joshua France, Mobile Systems Segment Manager, will be presenting 'Multisensor Precision in a Complex World: Automatic Adjustment Software' on Wednesday, April 16 from 9:00 AM to 10:30 AM; Broadmoor Hall D.

Tan Nguyen, Field Support Manager, and Dr. Dennis Hirota, President of Sam O. Hirota, will be presenting ‘RiALITY- Dive into the Point Cloud' on Wednesday, April 16 from 11:00 AM to 12:30 PM during the New Technologies- Augmented Reality session in Broadmoor Hall D. Bret Bienkowski, Terrestrial Segment Manager, will be presenting '4D Laser Scanning: Monitoring Change Over Time' on Thursday, April 17 from 9:45 AM to 10:15 AM; Broadmoor Hall F.

The introduction of the new Riegl VMZ, a high performance entry level mobile scanning system for combined 3D static and kinematic mobile scanning, will take place at the RIEGL USA booth, Booth 430, during SPAR International 2014. Be sure to stop by for more information! Riegl USA is proud to announce that NASA's Jet Propulsion Laboratory (JPL) has recently deployed their Riegl LMS-Q1560 airborne laser scanner system! NASA JPL is utilizing their Riegl LMS-Q1560 to more completely and accurately measure snowpack depth of major mountain watersheds. This will be accomplished through the use of innovative airborne LiDAR from Riegl.

NASA JPL's use of the Riegl LMS-Q1560 will allow them to better gauge the volume of water that travels down rivers and streams as runoff from snowpack. The data is then made accessible to state officials, water districts, power plant managers, and farmers to alert them to the possibility of flooding and water allocation. Riegl laser scanners have been sold successfully for several years in China through its distribution partners, Five Star Electronics and Red China Geosystems, both located in Beijing. To support the growing demand for Riegl products in China, Riegl now has established a Riegl Riegl brand in China. Riegl Riegl products in the Chinese market.

The company's focus will be to carry out marketing and sales related activities such as demo events, roadshows, training courses and attending major geospatial trade shows and conferences. The Beijing operations are also expected to achieve a better understanding of customer needs in China by receiving direct customer feedback in order to offer products and services that are tailored to local expectations. Riegl USA will be exhibiting at the GEOINT 2013* Symposium. GEOINT 2013* Symposium will be held at the Tampa Convention Center in Tampa, Florida from April 14 through April 17, 2014. RIEGL USA will also be exhibiting at Booth 7093 during the conference. Please be sure to come by the booth to talk to our staff about any questions that you may have about our hardware and software solutions for airborne, mobile, terrestrial, and our newest unmanned laser scanners!

RIEGL USA looks forward to a successful conference and to seeing you at the GEOINT 2013* Symposium! Riegl USA will be exhibiting and presenting at SPAR International 2014. SPAR International 2014 will be held at the Broadmoor in Colorado Springs, Colorado from April 14 through April 17, 2014. RIEGL USA will also be exhibiting at Booth 430 during the conference. Please be sure to come by the booth to talk to our staff about any questions that you may have about our hardware and software solutions!

RIEGL USA looks forward to a successful conference and to seeing you at SPAR International 2014. Riegl USA will be exhibiting and presenting at the ASPRS 2014 Annual Conference. The ASPRS 2014 Annual Conference will be held at the Galt House Hotel in Louisville, Kentucky from March 23 through March 28, 2014. RIEGL USA will also be exhibiting at Booth 601 during the conference. Please be sure to come by the booth to talk to our representative about any questions that you may have about our hardware and software solutions! Andres Vargas, Applications Systems Engineer will be presenting 'The New Era in Airborne Mapping: The RIEGL LMS-Q1560' on Wednesday from 1:30 PM to 3:00 PM during the LiDAR Technologies and Commercial Services Interactive Sessions.

RIEGL USA looks forward to a successful conference and to seeing you at the ASPRS Annual Conference. Riegl USA will be exhibiting at the 2014 SME Annual Meeting & Exhibit! The 2014 SME Annual Meeting & Exhibit will be held at the Salt Palace Convention Center in Salt Lake City, Utah from February 23 through February 26, 2014. RIEGL USA will also be exhibiting at Booth 739 during the conference. Please be sure to come by the booth to talk to our representative about any questions that you may have about our hardware and software solutions or to see demonstrations of our VZ-4000 terrestrial laser scanner! Riegl USA will be presenting and exhibiting at the International LIDAR Mapping Forum 2014.

The International LIDAR Mapping Forum 2014 will be held at the Hyatt Regency Denver at Colorado Convention Center in Denver, Colorado from February 17 through February 19, 2014. Martin Pfennigbauer, Director of Research and Intellectual Property of RIEGL Laser Measurement Systems, will be presenting 'New point attributes from online waveform processing' in the Data Fusion and Processing Technologies session on Tuesday, February 18, 2014 at 9:30 AM. RIEGL USA will be exhibiting at booth 36! Riegl USA will be presenting and exhibiting at the International LIDAR Mapping Forum 2014.

The International LIDAR Mapping Forum 2014 will be held at the Hyatt Regency Denver at Colorado Convention Center in Denver, Colorado from February 17 through February 19, 2014. Martin Pfennigbauer, Director of Research and Intellectual Property of RIEGL Laser Measurement Systems, will be presenting 'New point attributes from online waveform processing' in the Data Fusion and Processing Technologies session on Tuesday, February 18, 2014 at 9:30 AM. RIEGL USA will be exhibiting at booth 36! Riegl Laser Measurement Systems has been developing the world's first survey-grade UAS LiDAR sensor, the VUX-1. As early as February this year, it is already time to show off this innovative sensor: join us at the official launch event ILMF taking place in Denver, USA, February 17 to 19 where the VUX-1 will be presented for the first time! This VUX-1 will be on display during ILMF 2014 at the RIEGL USA booth, Booth 36. Stop by the booth during the show to see this innovative LiDAR sensor for yourself!

Riegl USA will be exhibiting at the 2013 54 th Annual Florida Division of the International Association for Identification (FDIAI) Conference. Nba 2k17 Crack Pc there. The 2013 54 th Annual FDIAI Conference will be held at the Miami Downtown Hilton in Miami, Florida from October 20 through October 24, 2013. RIEGL USA will be exhibiting at Booth 4 during the conference.

Please be sure to come by the booth to talk to our representative about any questions that you may have about our hardware and software solutions and see demonstrations with one of our VZ-400 terrestrial laser scanners! Riegl USA looks forward to a successful conference and to seeing you at the 2013 54 th Annual FDIAI Conference! SNCF, the national French railway society, has taken delivery of a RIEGL VMX-450-RAIL intended for the sole purpose of railway mapping. The complete scanning platform is either installed onto a mounting rack in the rear of a wagon or right into the engine cart.

The main tasks of railway mapping include geo-referencing of railway infrastructure, clearance monitoring in order to detect natural obstacles like vegetation, or determine infrastructural limits for special load transportation. Other applications of railway scanning include track and base terrain inspection, as well as supervision and registration of changes in train station structures, such as thresholds between platform and wagon due to renovation works in train stations. All these different tasks serve the enhancement of railway safety. Riegl USA will be exhibiting at the Florida Surveying and Mapping Society's (FSMS) 58 th Annual Conference. The FSMS 58 th Annual Conference will be held at the Tradewinds Resort St. Pete Beach in St. Petersburg, Florida from August 21 through 25, 2013.

Riegl USA will be exhibiting at Booth 24 during the conference. Please be sure to come by the booth to talk to our representative about any questions that you may have about our products or to see demonstrations of our VZ-400 terrestrial laser scanner. Riegl USA looks forward to a successful conference and to seeing you at the FSMS 58 th Annual Conference!

Riegl USA will be exhibiting at AUVSI's Unmanned Systems 2013 Conference. AUVSI's Unmanned Systems 2013 will be held at the Walter E. Washington Convention Center in Washington, D.C.

From August 12 through August 15. Riegl USA will be exhibiting at Booth 3854 during the conference. Please be sure to come by the booth to talk to our representative about any questions that you may have about our products.

Riegl USA looks forward to a successful conference and to seeing you at AUVSI's Unmanned Systems 2013! Riegl USA will be attending the ASPRS 2013 Annual Conference in Baltimore, Maryland. The conference will be held at the Baltimore Marriott Waterfront Hotel from March 24 through March 28, 2013. Peter Rieger, Product Manager, Airborne Laser Scanning, will be presenting 'Practical and Efficient Applications Utilizing Automated Range Ambiguity Resolution in High Repetition Rate Airborne LIDAR Systems' during the Automation session of ASPRS 2013 on Tuesday, March 26th between 1:3schie0 - 3:00 PM EST.

RIEGL USA is looking forward to a successful conference! Riegl USA will be attending the ILMF (International LiDAR Mapping Forum) 2013 Conference in Denver, Colorado. The conference will be held at the Hyatt Regency Denver at Colorado Convention Center in Denver from February 11 through February 13, 2013. Be sure to stop by our booth, booth #51, during the conference.

We also will have the Riegl VMX-450 on display in front of the building. We will have representatives from Riegl USA present at both the booth and the vehicle to answer any questions you may have about our products. Riegl USA Announces the Fully Automatic Multiple Time Around Processing Detection Software RIEGL fully automatic Multiple Time Around (MTA) zone detection workflow for the scanner. RiMTA is an automatic range ambiguity resolution algorithm which detects all MTA zones automatically during post processing. Multiple Time Around (MTA) processing allows multiple laser pulses to be in the air during data acquisition. The RiMTA improvement is especially beneficial for projects in the mountainous terrain, because it relaxes the requirements in flight planning.

RIEGL USA will host the first Riegl LiDAR 2012', in Orlando, Florida. The User Conference will be held on February 28 th thru March 1 st 2012 at the Hilton Orlando. Attendees will be offered three days of learning and networking sessions that cover various topics ranging from best practices to team building.

These intensive sessions will provide an opportunity to optimize current systems' investments through in-depth training and Q & A sessions. During this year's event, users will learn more about all the new and exciting instruments introduced by RIEGL at the INTERGEO 2011.

As infrastructure owners, operators, and managers strive to accomplish more with less money, surveying and software technologies are helping civil and structural engineers meet the challenge. 'Experts agree that more than $41 trillion will need to be spent on global infrastructure projects over the next 25 years,' said Terry Bennett, senior industry program manager for Civil Engineering and Planning at Autodesk. 'Many of these will be large, complex, high-risk projects set in confined urban environments that require a precise and accurate 3D spatial assessment of the existing infrastructure conditions.' A laser scan of a typical railway crossing, captured by a RIEGL VMX-250 system and shown here with false color reflectance, can provide information on sign and road surface conditions. RIEGL USA is proud to announce Surveying Solutions, Inc. (SSI) as a new Mobile Mapping User.

SSI will utilize the RIEGL VMX-250 with the CS6 Camera System to collect detailed topography, asset management, and precision survey data at near highway speeds. The use of the system will allow for safe non-hazardous working conditions. 'SSI is extremely pleased to be able to offer Mobile LiDAR (MoLi™) to our clients giving them an ability to utilize this phenomenal technology. After a thorough investigation into the technology and mobile mapping systems, the RIEGL VMX-250-CS6 proved to be the superior solution for SSI's surveying and mapping applications, and since taking delivery in April, we have successfully completed nearly two dozen projects with the system. SSI is proud to be the newest members of the already extensive RIEGL family, and we are looking forward to continuing to work with RIEGL' - Brian Bailey, Geospatial Services Director, SSI >. Since the great earthquake of Friday, March 11, 2011, and the disastrous tsunami that followed, RIEGL Austria has been closely observing the developments in Japan. The precarious situation at the nuclear power plants involved terrifies all of us.

At this very moment, we are concerned by the catastrophes that have taken place, and full of anxiety in view of danger yet ahead. Teruo Sasaki, CEO RIEGL Japan, keeps us informed of the current situation on site, that is, in Tokyo, where RIEGL Japan is headquartered. We pay our greatest respect to him, his family and his team – as to all Japanese people– for their calm and sobriety in managing this extremely demanding situation.

The new High Performance fully Calibrated VMX-250-CS6 Camera System that compliments the RIEGL VMX-250 Mobile Laser Scanning System, by supporting six digital color cameras, has arrived in the United States. This new system is extremely compact and user-friendly. As seen in this release, the RIEGL VMX-250-CS6 is mounted on a lightweight aluminum frame designed for versatility and stability. RIEGL's RiAcquire Software manages acquisition of precisely time stamped images by the VMX-250-CS6 in addition to the acquisition of the VMX-250 laser scanner data. Accordingly, the software controls the camera's parameters and enables image monitoring during acquisition in real time.

RIEGL's RiProcess software then delivers high quality colorized point clouds.

Microdeletions in the AZF region of the Y chromosome are among the most frequent genetic causes of male infertility, although the specific role of the genes located in this region is not fully understood. AZFa and AZFb deletions impair spermatogenesis since no spermatozoa are found in the testis. Deletions of the AZFc region, despite being the most frequent in azoospermic patients, do not correlate with spermatogenic failure. Therefore, the aim of this work was to develop a screening method to ascertain the presence of the main spermatogenesis candidate genes located in the AZFc region in the light of the identification of those responsible for spermatogenic failure. DAZ, CDY, BPY2, PRY, GOLGA2LY and CSGP4LY genes were selected on the basis of their location in the AZFc region, testis-only expression, and confirmed or predicted protein codification.

AMEL and SRY were used as amplification controls. The identification of Real Time PCR products was performed by High Resolution Melting analysis with SYTO 9 as intercalating dye. The herein described method allows a rapid, simple, low-cost, high-throughput screening for deletions of the main AZFc genes in patients with spermatogenic failure.

This provides a strategy that would accelerate the identification of spermatogenesis candidate genes in larger populations of patients with non-obstructive idiopathic azoospermia. Introduction Infertility affects about 15% of the couples trying to conceive. In half of the cases, the underlying cause can be partially or totally attributed to the male partner, and 10–15% of the cases could be due to genetic abnormalities.

For many years, microdeletions in the euchromatic region of the Y chromosome have been linked to spermatogenic failure, as cytogenetic studies associated a deletion on its long arm (Yq) to azoospermia. The azoospermia factor region (AZF) and its sub-regions AZFa, AZFb and AZFc are the main targets for molecular diagnosis,. These AZF loci are routinely screened for possible microdeletions using sequence tagged sites (STSs), as recommended by the European Academy of Andrology (EAA). This diagnostic testing of Yq microdeletions is generally performed by PCR amplification of selected STSs on the Y chromosome. The precise sequencing and mapping of the male-specific region of the Y chromosome clarified that many STSs used in initial screening were polymorphic, ambiguous, heterogeneous and unreliable, prone to produce technical problems and false results,. The use of gene-specific primers flanking specific locations enables to obtain information on the potential mechanisms, and the extension of the deletions.

These studies also highlighted the improved value of including gene-specific markers in the minimal set of markers for the analysis. On the other hand, complete deletions of specific AZF regions have been associated with different spermatogenic scenarios such as: AZFa to Sertoli cell-only syndrome, AZFb/AZFbc to spermatogenic arrest with interruption in meiosis I, and AZFc to hypospermatogenesis leading to severe oligozoospermia and azoospermia. Interestingly, microdeletions of either the entire AZFa or AZFb regions heralds a poor prognosis for sperm retrieval from testicular sperm extraction (TESE) or testicular biopsy, whereas half of the men with complete AZFc deletion have successful sperm retrieval. Nonetheless, the AZFc region is the most frequently deleted in patients with non-obstructive azoospermia or severe oligozoospermia, attaining about 70% of all the cases. From previous observations, neither it is possible to infer the phenotype from the classic genetic testing for the AZFc region nor to identify the genes essential for normal spermatogenesis that this sub-region harbors.

Hence, in order to provide a strategy that would accelerate the identification of spermatogenesis candidate genes, our goal was to develop a method that would allow the screening of the main AZFc genes in large number of patients with non-obstructive idiopathic azoospermia or severe oligozoospermia. In terms of gene content, the AZFc region contains 11 families of transcription units expressed only in the testis. Five of them code for proteins, four are pseudogenes, and two have open reading frames. Namely, the deleted in azoospermia ( DAZ) gene family is expressed in spermatogonia and encodes a RNA-binding protein important for spermatogenesis. Four copies of this gene ( DAZ1, DAZ2, DAZ3 and DAZ4) are located in AZFc,,. The chromodomain protein Y ( CDY) gene family encodes a protein containing a chromodomain and a histone acetyltransferase catalytic domain located in the nucleus of late spermatids where histone hyperacetylation takes place. The human Y chromosome has two identical copies of this gene within the AZFc region ( CDY1A and CDY1B) and a pair of closely related genes in the palindrome P5 ( CDY2A and CDY2B),.

The testis-specific basic protein Y 2 ( BPY2) gene is expressed specifically in testis and its protein product is involved in male germ cell development and male infertility. Three nearly identical copies of this gene exist on Y chromosome. The PTPBL-related gene on Y ( PRY) is expressed specifically in testis and encodes a protein with similarity to protein tyrosine phosphatase, non-receptor type 13. Two nearly identical copies of this gene exist on the Y chromosome.

Golgi autoantigen, golgi subfamily a, 2-like, Y-linked ( GOLGA2LY) and chondroitin sulfate proteoglycan 4 pseudogene 1, Y-linked ( CSPG4P1Y) families of transcription units have significant open reading frames, are expressed only in testis and have predicted protein products. Both have 2 copies located in P1 palindrome. The previous are the main protein-coding genes located in the AZFc region that are potentially related to spermatogenic failure, although the specific contribution to spermatogenesis of each of the individual genes is still not fully understood. Aiming to clarify the implication of the main candidate genes in spermatogenic failure, we selected these genes for a deeper understanding of how Y chromosome microdeletions, and specifically the deletion of AZFc-located genes, affect normal spermatogenesis. Herein we designed a one-step multiplex PCR reaction for the identification of the complete absence of six AZFc genes. The developed method allows a rapid, simple, low-cost, high-throughput screening for deletions of the main AZFc genes in patients with non-obstructive idiopathic azoospermia. Design of the multiplex Real Time PCR/HRM method As a first approach in order to develop a multiplex Real Time PCR (rtPCR) system with High Resolution Melting analysis (HRM) for AZFc gene screening we designed primers for the specific amplification of all the copies of six AZFc genes.

The following genes were selected on the basis of their location in the AZFc region of the Y chromosome, testis-only expression and confirmed or predicted protein codification: DAZ, CDY, BPY2, PRY, GOLGA2LY and CSGP4LY. Furthermore, SRY was selected as internal amplification control, and AMELxy as internal and specificity control. These primers were combined to establish one single 8-plex reaction. High peak discrimination was achieved by selecting amplification products with different predicted melting temperature (Tm) values for each peak. As a first step, we amplified normal undeleted male samples as well as female samples attaining high amplification levels for all the selected genes in male samples, while no amplification (except for AMELxy) was observed in female samples (). This optimal condition was achieved by increasing the annealing temperature of the PCR to 62°C.

In order to confirm the specificity of the amplification, all PCR products were sequenced and aligned to the GeneBanks reference sequence, matching the genes of interest. Performance of the method to detect specific gene deletions We tested control samples with AZFa, AZFb, AZFbc and AZFc deletions in order to characterize the ability of the system to identify the specific deletion of the genes located in these regions. Deleted samples were previously analyzed by a modified for Real Time EAA protocol in order to detect classical AZF microdeletions and then undeleted samples were further analyzed for partial AZFc microdeletions by means of STSs analysis, including sY142, sY1191, sY1197, sY1201, sY1206, sY1261 and sY1291 markers,. As expected, inferred from the GenBanks Y chromosome reference sequence, the AZFa-deleted sample evidenced no deletion of the analyzed genes, conversely to AZFb-deleted that showed lack of amplification for PRY gene, AZFc-deleted no amplification of BPY2, DAZ, GOLGA2LY and CSGP4LY genes, and AZFbc-deleted no amplification of PRY, BPY2, DAZ, GOLGA2LY and CSGP4LY genes. Only those samples with complete AZFabc deletion were negative for all the genes, while positive for AMEL and SRY amplification controls (). When HRM data analysis was performed with Rotor Gene software, which allows the automatic identification of the peaks in the derivative (dF/dT) plot, the assignment of the sample to a specific genotype was enabled. High Resolution Melting derivative (dF/dT) profile for deleted and undeleted samples.

Furthermore, we analyzed samples typified with Y chromosome STSs markers routinely employed to identify gr/gr, b1/b3 and b2/b3 partial AZFc microdeletions. As expected, no peak loss was observed as a consequence of the remaining gene copies.

Although no diagnosis of partial AZFc deletions could be performed by this technique, a variation of peak height was observed in partially deleted samples, most likely due to the decrease of the remaining gene copy number. For example, in b2/b3 deleted samples an inversion of peak height was observed for BPY2, CSGP4LY and GOLGA2LY genes, which are located within the deleted region (). The same effect was observed in samples with an AZFb deletion, where a decrease in peak height for CDY, BPY2 and DAZ was observed as a consequence of gene copy reduction. Performance of three different intercalating dyes for multiplex HRM As the amplification was tested with three intercalating dyes, namely SYBR Green I, EvaGreen and SYTO 9, it was possible to compare their performance in multiplex amplification and HRM peak discrimination.

At first, the 8-plex reaction was set up with SYTO 9 achieving high sensitivity, optimal melting peak resolution and correlation between predicted and real Tm. When the same reaction was reproduced with SYBR Green I, sensitivity was compromised obtaining about 30% less fluorescence than with SYTO 9 and peak loss was observed (; green). Likewise, when the reaction was performed with EvaGreen, a shift in the predicted melting peaks towards higher temperatures was observed (; blue).

Moreover, both tested commercial kits are more expensive and require more DNA and primer input, in contrast to SYTO 9 that allowed a fast setup of the multiplex reaction with minimal costs. Therefore, we designed a standard homemade PCR master mix with a universal intercalating dye as SYTO 9 and regular desalted primers, plus DNA and primer concentrations used have been reduced to a minimum without losing sensitivity. Robustness and sensitivity To test the performance of the multiplex for robustness regarding peak strength and separability, we analyzed 10 different genomic DNA samples from males without AZFc deletions. Regardless the origin of the sample, the melting peaks from every sample were present and could be clearly distinguished. Furthermore, robustness was tested by a blind assay performed by a different co-worker in which 5 undeleted controls and three samples from AZFc deleted patients (b2/b4) were analyzed. In agreement with the corresponding deleted region, the AZFc deleted samples were correctly identified as missing BPY2, DAZ, GOLGA2LY and CSGP4LY genes (data not shown).

The sensitivity of the system was evaluated by using genomic DNA from an undeleted control. We performed the PCR reaction with two-fold dilutions of the control sample ranging 20–0.312 ng/µL. The individual melting peaks could be accurately identified at concentrations bellow 10 ng/µL, despite that no linear correlation between DNA concentration and signal strength was evidenced.

It is worth to notice that despite the complexity of the multiplex reaction, all samples extracted by semi-automated methods (usually ranging from 1 to 10 ng/µL) could be easily analyzed, even avoiding previous quantification. Portability of the method to a 96-well block based system As the multiplex system was established on a rotary with moving hot air-based heating system Real Time PCR equipment (Rotor Gene 6000), it was worthy to check portability of the method to a different equipment. Hence, the reaction was also tested on a 96-well block based system (StepOne Plus, Life Technologies, USA).

The latest is based on a heating block with LED based 4-color optical detection. The multiplex reaction was reproduced exactly as it was set up for Rotor Gene with the unique modification of the melting curve stage following the manufactureŕs indications. We also obtained an optimal peak separability, although the analysis with StepOne Plus software was only qualitative due to the limitation of the software to assign multiple melting peaks, hampering the simultaneous analysis of all the genes in a high number of samples (). Clinical validation of the method The validation of the method was performed by analyzing 140 samples from patients consulting for infertility (screening group), and 90 samples from azoospermic patients that were candidates for testicular biopsy (azoospermic group). In the screening group, only one patient with a complete AZFc or b2/b4 deletion showed the absence of DAZ, GOLGA2LY, CSGP4LY and BPY2 genes. In the azoospermic group, those with complete deletions of either or both AZFb or AZFc regions were lacking the genes located in these regions, namely DAZ, GOLGA2LY, CSGP4LY and BPY2 in AFZc, plus PRY in AZFb. One sample with a complete AZFabc deletion showed the absence of all the analyzed genes.

All the samples with gene deletions were azoospermic or severely oligozoospermic, and none of the samples with sperm count above 5×10 6/mL evidenced any gene deletion. Multiplex PCR conditions Multiplex amplification was tested with three intercalating dyes included in different multiplex reactions, namely SYTO 9, SYBR Green I (Life Technologies, USA) and EvaGreen (Biotium, USA).

SYBR Green I was part of the Quantitec SYBR Green PCR kit (QIAGEN, Germany) and EvaGreen of Mezcla Real (Biodynamics, Argentina). Commercial kit protocols were performed as suggested by the manufacturer. Meanwhile, the herein developed protocol employing SYTO 9 as intercalating dye was performed as follows. Multiplex PCR reaction was carried out in 25 µL final volume containing 37.5 pmol SYTO 9, 2 units GoTaq DNA Polymerase (Promega, USA), 5× Colorless GoTaq Reaction Buffer (Promega, USA), 20 nmol 4 dNTPs mix (Promega, USA), primers (Integrated DNA Technologies, USA) (as indicated in ) and 2 µL DNA template (with a concentration between 0.3125 and 10 ng/µL).

A detailed protocol is described in (see Supporting Information). Primer sequences and concentrations used in all the reactions are listed in. The reactions were performed in a Rotor Gene 6000 (Corbett Life Science, Australia) and in a StepOne Plus (Life Technologies, USA) Real Time PCR equipment.

PCR cycling conditions were the following: initial denaturation step at 95°C for 1 min followed by 30 cycles of 94 °C 10 s/62 °C 30 s/72 °C 30 s. Following amplification, a High Resolution Melting (HRM) analysis was performed ranging from 74 °C to 91 °C at 0.2 °C/s for Rotor Gene 6000. When using StepOne Plus, the melting curve stage was set as follows: 95°C 15 s–60°C 1 min followed by a 0.3% continuous ramp from 74 °C to 91 °C. Likewise, the cycling profile has been modified when Hot Start polymerase was used, as with Quantitec SYBR Green PCR kit. Sequencing of the amplification products For confirmation of the specificity of the amplification, all PCR products were cleaned up using Illustra ExoStar Kit (GE Healthcare, UK) and sequenced with both forward and reverse primers using the Big Dye Terminator Cycle Sequencing kit v3.1 (Life Technologies, USA), according to the supplier's protocol. Purification of the sequences from residual dye terminators was performed by Isopropyl alcohol 75%/Ethanol 70% precipitation.

Capillary Electrophoresis of the sequences was carried out in an ABI3500 Genetic Analyzer (Life Technologies, USA). Electropherograms were visualized and edited with Sequencher v4.8 software (Gene Codes Corporation, USA).

The obtained sequences were aligned with GeneBanks reference sequence. Analysis of classical AZF microdeletions Classical AZF microdeletions were analyzed by the EAA protocol, modified for Real Time PCR. Briefly, AZFa, AZFb, AZFc and AMEL primers were used.

The protocol was adapted and setup for amplification with SYTO 9 as intercalating dye. From the primer combinations described by Kozina et al. We selected those from Setup 2 (AMELxy, sY255, sY127 and ), with the exception of primer that, due to unspecific amplification of a sequence located on the X chromosome, was switched for sY85. Figure S1 High Resolution Melting derivative (dF/dT) profile testing the performance of SYTO 9, SYBR Green I and EvaGreen. The figure shows the bin locations according to standard amplification with SYTO 9 of an undeleted sample (red).

EvaGreen shows optimal amplification of all the genes but with approximately 1°C shift in melting temperature (blue). SYBR Green I shows lack of detection of BPY2 genes, shift in melting temperature and imbalance in melting peak height within all the genes (green). The comparison was performed in standard amplification conditions and 10 ng of DNA input.